منابع مشابه
Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.
Highly purified HindIII restriction fragments of Xenopus laevis 5S DNA and of Psammechinus miliaris histone DNA have been covalently inserted into a derivative of phage lambda. This phage, genetically constructed by Murray et al. (1), contains only a single target for HindIII in the cI gene. Viable hybrid molecules were detected as clear plaque-forming phage after transfection of E. coli, the v...
متن کاملStability puzzles in phage lambda.
In the absence of RecA-mediated cleavage of the repressor, the lambda prophage is exceptionally stable. We develop a stochastic model that predicts the stability of such epigenetic states from affinities of the molecular components. We find that the stability, in particular, depends on the maximum possible cI protein production, and on the number of translated cro proteins per transcribed mRNA....
متن کاملMapping of integration and excision crossovers in superinfection double lysogens for phage lambda in Escherichia coli.
I N a previous paper it was shown that one prophage apparently acts as a homology region for a superinfecting phage of the same kind (CALEF, MARCHELLI, GUERRINI 1965). A more rigorous test for the same possibility can be obtained by correlating the frequency of integration of superinfecting phage at any given region with the map length of that region. The work presented here consists primarily ...
متن کاملMechanistic insights into filamentous phage integration in Vibrio cholerae
Vibrio cholerae, the etiological agent of acute diarrhoeal disease cholera, harbors large numbers of lysogenic filamentous phages, contribute significantly to the host pathogenesis and provide fitness factors to the pathogen that help the bacterium to survive in natural environment. Most of the vibriophage genomes are not equipped with integrase and thus exploit two host-encoded tyrosine recomb...
متن کاملDomestication of Lambda Phage Genes into a Putative Third Type of Replicative Helicase Matchmaker
At the onset of the initiation of chromosome replication, bacterial replicative helicases are recruited and loaded on the DnaA-oriC nucleoprotein platform, assisted by proteins like DnaC/DnaI or DciA. Two orders of bacteria appear, however, to lack either of these factors, raising the question of the essentiality of these factors in bacteria. Through a phylogenomic approach, we identified a pai...
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ژورنال
عنوان ژورنال: The Japanese Journal of Genetics
سال: 1983
ISSN: 0021-504X,1880-5787
DOI: 10.1266/jjg.58.95